Skip to main content
. Author manuscript; available in PMC: 2022 Apr 14.
Published in final edited form as: Biomacromolecules. 2019 Aug 22;20(9):3352–3365. doi: 10.1021/acs.biomac.9b00133

Figure 7.

Figure 7

Osteogenesis of pre-osteoblast cell and rabbit spine injection. a) Intracellular ALP, b) OCN and c) total collagen production of MC3T3 pre-osteoblast cells cultured on crosslinked PFCL-Click scaffolds with α-MEM medium for 1, 2, and 3 weeks. After culture in an osteogenic medium supplemented with 10 mM β-glycerophosphate and 50 μg/mL ascorbic acid for 1, 2, 3 weeks, the d) intracellular ALP, e) OCN and f) total collagen production of MC3T3 pre-osteoblast cells were also determined. g) Photographs of PPF-BCN mixed with hyPCL32-N3 and incorporated with ZrO2. h) X-ray images of the PFCL-Click/ZrO2 system after injection to the rabbit vertebral bodies. *: Statistically different (p < 0.05).