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M9 Medium
| Component | Stock volume |
|---|---|
| 19aa (5x) | 250 mL |
| Proline (5x)* | 250 mL |
| M9 salts (5x) | 250 mL |
| Glucose (10x) | 125 mL |
| Biotin (100x) | 1.25 mL |
| Thiamine (1,000x) | 1.25 mL |
| FeSO4 (1,000x) | 1.25 mL |
| MgSO4 (1,000x) | 1.25 mL |
| CaCl2 (1,000x) | 1.25 mL |
| Trace metals (10,000x) | 125 μL |
| Water | 365 mL |
| Total | 1.25 L |
Omit 5x proline solution for 1.25x 19aa M9 preparation
