TABLE 3.

Behavioral, structural, and molecular impacts following combined adolescent social isolation and adolescent ethanol exposure.

Study	Strain (sex)	Isolation model (age of isolation onset)	EtOH paradigm	Behavioral impacts	Structural impacts	Molecular impacts
Sanna et al., 2011	C57BL/6J mice (M)	GH (6/cage) or SI (PND 21)	c-2BC, 15% EtOH (only for EtOH intake experiment)	↑Intake and pref due to SI, ↓locomotion due to SI (OF, 9 weeks)		↑α4 and δ GABA mRNA subunits in hippocampus due to SI (9 weeks)
Schenk et al., 1990	Long-Evans rats (M)	GH (4/cage) or SI (PND 21 or PND 65)	c-2BC, 10% EtOH	↑Intake and pref due to SI
Pisu et al., 2011b	Sprague-Dawley rats (M)	GH (3/cage) or SI (PND 25)	24 h access 2BC, 4 weeks, 2.5–10% EtOH, PND 25–53	↓Pref due to SI, ↑anxiety-like behavior (EPM)		↑α4 (SI) and δ (SI and EtOH) GABA mRNA subunits in hippocampus

Drinking data presented as (drinking paradigm, length of drinking period, percentage EtOH, PND drinking began) unless specific PNDs were given for drinking period. GH, group-housed; SI, social isolation; PND, postnatal day; M, males; F, females; i.p., intraperitoneal; i.g., intragastric; BC, bottle choice; c-2BC, consecutive 2-bottle choice; pref, preference; OF, open field; EPM, elevated plus maze.