FIGURE 7.

Reactivity of different antibodies to Arctic Aβ in App^NL–G–F mice. (A) Epitope map of anti-Aβ antibodies. (B,C) Quantification of Arctic Aβ species using BNT77 as a capture antibody. BNT77 binds to the mid-portion of Aβ [see epitope map (A)]. A sandwich ELISA kit (Wako, Japan) was used to quantify Aβx-40 (C) and Aβx-42 (D), respectively. (D,E) Quantification of Arctic Aβ species using BAN50 as a capture antibody. BAN50 binds to the N-terminal region of Aβ [see epitope map (A)]. A sandwich ELISA kit (Wako, Japan) was used to quantify Aβx-40 (D) and Aβx-42 (E), respectively. BNT77 and BAN50 captured Arctic Aβ more weakly than wild-type Aβ. (F) Immunohistochemistry using various anti-Aβ antibodies. Brain sections derived from App^NL–F mice (24 months old) were immunostained using antibodies with different epitopes after antigen retrieval as indicated (upper panels); those of App^NL–G–F mice (9 months old) were similarly immunostained (lower panels). Scale bars represent 500 μm.