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. 2022 Mar 31;13:867962. doi: 10.3389/fimmu.2022.867962

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Copyright © 2022 Koelle, Dong, Jing, Laing, Zhu, Jin, Selke, Wald, Varon, Huang, Johnston, Corey and Posavad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Responses of bulk-expanded, AIM-selected TCL from subject 15018 June cervix biopsy. (A) CD4 T cells. Top row shows gating scheme. Autologous PBMC used as APC were dump-gated by prior labeling with CTV. Lower row has gated CD4 T cells analyzed by ICS for accumulation of IFN-γ and IL-2 with the labeled stimuli. Specific responses are present to autologous PBMC treated with UV-HSV-2 antigen for CD4 responder cells. (B) CD8 T cells. Upper row has gating scheme. EBV-LCL used as APC are CD3-negative and did not require dump gating. Lower row shows responses to autologous EBV-LCL infected with HSV-2 or mock virus, and control stimuli. Numbers are percentages of gated cells.