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. 2022 Mar 31;13:867962. doi: 10.3389/fimmu.2022.867962

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Copyright © 2022 Koelle, Dong, Jing, Laing, Zhu, Jin, Selke, Wald, Varon, Huang, Johnston, Corey and Posavad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HSV-2 FRT CD4 T cell antigen and epitope discovery using culture-expanded T_RM. (A) Screen of skin biopsy-derived TCL from subject 9149 against HSV-2 proteome in duplicate proliferation assay. Gene and short protein names of antigenic proteins are shown; negative control mock virus and positive control whole UV-killed HSV-2 and PHA at right. (B) Peptide epitope workup for cervix-derived TCL from subject 9149. OLP from HSV-2 gD (gene US6, 15AA long, left) or HSV-2 VP22 (gene UL49, 13 AA long, right) were arrayed in row (RP) and column (CP) pools with 1 μg/ml each peptide. Peptides from the intersection (UL6) or intersections (UL49) of reactive pools were re-assayed at 1 μg/ml at right of each diagram. APC were autologous irradiated PBMC. Colored dots are duplicate raw data and gray bar is mean.