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. 2022 Feb 22;5(1):109–122. doi: 10.1089/crispr.2021.0085

Table 1.

Primer Sequences for PCR, Sanger, and Deep Sequencing

Sanger sequencing of APP exon 16 TCAAGCCTGTAATCCCAGCA
Sanger sequencing of EMX1 exon 3 TGAGTTTCTCATCTGTGCCC
PCR of APP exon 16 Fwd AAATCCTTGCCAACCTCTCAACCA
PCR of APP exon 16 Rev GTTCTTTCAGACTACAAGGCAGCA
PCR of EMX1 exon 3 Fwd CAGCTCAGCCTGAGTGTTGA
PCR of EMX1 exon 3 Rev CTCGTGGGTTTGTGGTTGC
Deepseq of APP exon 16 Fwd ACACTGACGACATGGTTCTACA
GGTAGGCTTTGTCTTACAGTGTTA
Deepseq of APP exon 16 Rev TACGGTAGCAGAGACTTGGTCT
TGGTAATCCTATAGGCAAGCATTG
Deepseq of APP exon 17 Fwd ACACTGACGACATGGTTCTACA
ATCCAAATGTCCCCTGCATTTAAG
Deepseq of APP exon 17 Rev TACGGTAGCAGAGACTTGGTCT
TTTACCTACCTCCACCACACCATG
Deepseq of EMX1 exon 3 Fwd ACACTGACGACATGGTTCTACA
CAGCTCAGCCTGAGTGTTGA
Deepseq of EMX1 exon 3 Rev TACGGTAGCAGAGACTTGGTCT
CTCGTGGGTTTGTGGTTGC

PCR, polymerase chain reaction.