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Sequences of sgRNAs Used in Experiments
| sgRNA | Spacer sequence |
|---|---|
| sgRNA −138 | CCTACCCAAAACTTCTTTCT |
| sgRNA −69 | ACATCATAATTAAAGTATGC |
| sgRNA +79 | AGACAAACAGTAGTGGAAAG |
| sgRNA +131 | CTTAATTTGATTTCTAGCAC |
| sgRNA_London +48 | AGGTGCAATCATTGGACTCA |
| sgRNA_PAM2 − 92 | GCCAAATGACCTATTAACTC |
| sgRNA_PAM2 + 72 | CTTTAATTATGATGTAATAC |
| sgRNA_PAM2 PE3b | ACAGAATTCCGACATGACTC |
| sgRNA_PAM2 PE3b_pm | ACAGAATTCAGACATGACTC |
sgRNA, single-guide RNA.
