Fig. 1. IL-12-Fc fusion proteins potently control tumor growth.
(A) Schematic structure of hetero-IL-12-Fc and homo-IL-12-Fc.
(B) Activity of fusion proteins as detected by the Hek-Blue™ IL-12 reporter cell line. Reporter cells were cultured with serial dilutions of each protein for 24 hours. In the cell culture supernatant, secreted embryonic alkaline phosphatase (SEAP) was detected by reading the OD value at 630nm with a microplate reader.
(C-D) Tumor growth curves of C57BL/6N mice inoculated with 5×105 MC38 cells and then teated with indicated doses of homo-IL-12-Fc (n=5/group): 66.7pmol, 13.3pmol, 6.67pmol. PBS (n=5) (C). Hetero-IL-12-Fc: 6.67pmol (n=14), 1.33pmol (n=13), 0.27pmol (n=10). PBS (n=14) (D) by i.p. injection three times (days 13, 16, and 19). Tumor size was measured twice per week.
(E) Tumor growth curves of C57BL/6N mice (n=9/group) inoculated with 5×105 MC38 cells and then treated with PBS, 6.67pmol homo-IL-12-Fc or hetero-IL-12-Fc fusion protein by i.p. injection on days 13, 16, and 19.
(F) Tumor growth curves of C57BL/6N mice (n=9/group) inoculated with 3×105 B16-F10 cells and then treated with PBS, 33.3pmol hetero-IL-12-Fc or homo-IL-12-Fc fusion protein by i.p. injection on days 10, 13, and 16.
Data indicate mean ± SEM and are repeated two or three independent experiments. Statistical analysis for E and F was performed using A two-way analysis of variance (ANOVA).*P <0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.