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. 2022 Apr 13;11(1):2062827. doi: 10.1080/2162402X.2022.2062827

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — A) Flow cytometry data of CD40 expression on mouse bone marrow derived myeloid cells treated with a non-targeting (NT) control siRNA, CpG- NT siRNA, or CpG-OPN siRNA. B) IsoPlexis analysis of the cytokine secretome from human PBMC CD11b+ myeloid cells (n = 2) treated with CpG-NT siRNA or CpG-OPN siRNA. The most deregulated cytokines, GM-CSF, IL-18, and MIF, were plotted as a bar graph (p < .0001 for all). RFU: Relative fluorescence unit. C) Quantification of OPN and GAPDH mRNA in CpG-NT or CpG-OPN treated CD11b+ cells prior to the IsoPlexis analysis.