Table 3.

Effect of capsaicin on shear-induced Na_V1.5 mechanosensitivity in whole cells. Effects of shear stress (0 or 10 mL/min) on parameters of whole cell Na⁺ currents without (0 µM) or with capsaicin (20 µM): maximum peak conductance (G_MAX), maximum peak current density (I_PEAK), voltage dependence of activation (V_1/2A) or inactivation (V_1/2I), time constants of activation (τ_A) and fast (τ_F) or slow inactivation (τ_S), time of inactivation recovery (t_1/2R), slope of inactivation recovery (slope), maximum use-dependent inhibition (block), frequency of use-dependent inhibition (ƒ_1/2). n = 8–18 cells, *P < 0.05, 0 to 10 mL/min or †P < 0.05, 0 to 20 µM capsaicin by a 2-way ANOVA with Tukey posttest

	0 mL/min	0 µM 10 mL/min	Change	n	0 mL/min	20 µM 10 mL/min	Change	n
GMAX (nS)	0.98 ± 0.13	1.24 ± 0.22*	0.26 ± 0.10	12	0.78 ± 0.12†	0.85 ± 0.15†	0.08 ± 0.05	12
IPEAK (pA/pF)	−66.8 ± 12.3	−86.6 ± 17.7*	16.0 ± 3.1%	12	−56.7 ± 58.5†	−58.5 ± 11.6†	3.1 ± 3.8%†	12
V1/2A (mV)	−57.3 ± 1.7	−58.9 ± 1.4*	−1.5 ± 0.6	12	−58.9 ± 1.5†	−59.2 ± 1.6	−0.3 ± 0.1	12
V1/2I (mV)	−90.3 ± 3.3	−92.7 ± 2.6*	−2.5 ± 0.9	12	−95.4 ± 2.6†	−95.8 ± 2.7†	−0.5 ± 0.6	12
τA (ms)	0.5 ± 0.05	0.39 ± 0.02*	−20.4 ± 3.3%	12	0.39 ± 0.04†	0.38 ± 0.04	−1.3 ± 7.0%†	12
τF (ms)	0.82 ± 0.08	0.57 ± 0.04*	−27.5 ± 4.0%	12	0.80 ± 0.08	0.56 ± 0.05*	−25.1 ± 5.9%	12
τS (ms)	4.7 ± 0.3	3.5 ± 0.2*	−24.2 ± 4.4%	12	3.8 ± 0.3†	2.9 ± 0.2*†	−20.5 ± 4.8%	12
t1/2R (ms)	18.8 ± 1.7	16.6 ± 1.8*	−2.2 ± 0.6	8	38.0 ± 4.5†	28.4 ± 4.2*†	−9.5 ± 0.9†	8
slope (ms−[1])	−1.26 ± 0.04	−1.28 ± 0.05	−0.02 ± 0.02	8	−1.43 ± 0.02†	−1.42 ± 0.06	0.01 ± 0.04	8
Block (%)	64.6 ± 2.4	64.7 ± 2.6	0.2 ± 1.7	18	89.0 ± 1.4†	83.9 ± 2.0*†	−5.1 ± 0.9†	10
ƒ1/2 (Hz)	26.1 ± 2.0	27.3 ± 2.3	1.2 ± 0.9	18	18.9 ± 1.0†	21.0 ± 1.7	2.1 ± 1.4	10