FIG. 1.
(A) Agarose gel electrophoresis of the plasmid pPM14, purified from P. mirabilis FI-14. Lanes: U, uncut; H, B, R, V, P, after digestion with HindIII, BglII, EcoRI, EcoRV, and PstI, respectively. (B) Results of Southern blot analysis of the gel shown in panel A using the blaTEM probe. DNA size standards (in kilobase pairs) are shown on the left. Identical results were obtained with plasmid pMN-13 purified from M. morganii FI-13 and with plasmids purified from the respective E. coli transformants.