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Summary of the purification steps of TEM-72 β-lactamase from E. coli DH5α(pPM-14)
| Purification step | Total protein (mg) | Sp act (U/mg protein)a | Total activity (U) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 980 | 1.4 | 1,372 | 1 | 100 |
| Q-Sepharose FF | 120 | 8 | 960 | 5.7 | 70 |
| Superdex G75 FF | 36 | 21 | 756 | 15 | 55 |
| Fast chromatofocusing | 3.2 | 136 | 435 | 97 | 32 |
Measured against cefotaxime 100 μM; 1 U is defined as the activity that hydrolyzed 1 μmol of the substrate/min.
