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. 2022 Mar 31;9:851987. doi: 10.3389/fvets.2022.851987

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Copyright © 2022 Ndiana, Lanave, Vasinioti, Desario, Martino, Colaianni, Pellegrini, Camarda, Berjaoui, Sgroi, Elia, Pratelli, Buono, Martella, Buonavoglia and Decaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree based on alignment of the partial replicase gene (373 nt) of canine cyclovirus isolates detected in this study and other cycloviruses retrieved from the GenBank database. Horse cyclovirus (GenBank accession no. KR902499) was used as outgroup. Posterior output of the tree was derived from Bayesian inference using 4 chains run for >1 million generations, 2-character states (Hasegawa–Kishino–Yano) model, a proportion of invariable sites and a subsampling frequency of 1,000. Posterior probability values >95 are indicated on the tree nodes. The black arrows indicate the sequences identified in this study. Scale bar indicates nucleotide substitutions per site.