REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Casirivimab	Laboratory of Hans-Martin Jäck	N/A
Imdevimab	Laboratory of Hans-Martin Jäck	N/A
Bamlanivimab	Laboratory of Hans-Martin Jäck	N/A
Etesevimab	Laboratory of Hans-Martin Jäck	N/A
hIgG	Laboratory of Hans-Martin Jäck	N/A
Goat anti-Human IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11013; RRID: AB_2534080
Goat anti-Human IgG (Fc)-HRPO	Dianova	Cat# 109-035-098; RRID: AB_2337586
Anti-VSV-G antibody (I1, produced from CRL-2700 mouse hybridoma cells)	ATCC	Cat# CRL-2700; RRID: CVCL_G654
Bacterial and virus strains
VSV∗ΔG-FLuc	Laboratory of Gert Zimmer	N/A
One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli	Thermo Fisher Scientific	Cat# C854003
Biological samples
Convalescent plasma (ID15)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID18)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID20)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID22)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID23)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID24)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID27)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID33)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID51)	Laboratory of Martin S. Winkler	N/A
Convalescent plasma (ID56)	Laboratory of Martin S. Winkler	N/A
Vaccinee serum (L3)	Laboratory of Martin Lier	N/A
Vaccinee serum (L4)	Laboratory of Martin Lier	N/A
Vaccinee serum (L9)	Laboratory of Martin Lier	N/A
Vaccinee serum (L11)	Laboratory of Martin Lier	N/A
Vaccinee serum (L12)	Laboratory of Martin Lier	N/A
Vaccinee serum (L13)	Laboratory of Martin Lier	N/A
Vaccinee serum (L16)	Laboratory of Martin Lier	N/A
Vaccinee serum (L22)	Laboratory of Martin Lier	N/A
Vaccinee serum (L25)	Laboratory of Martin Lier	N/A
Vaccinee serum (L26)	Laboratory of Martin Lier	N/A
Vaccinee serum (ID6205)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6272)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6236)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6239)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6243)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6262)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6297)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6321)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6358)	Laboratory of Georg M. N. Behrens	N/A
Vaccinee serum (ID6365)	Laboratory of Georg M. N. Behrens	N/A
Chemicals, peptides, and recombinant proteins
Soluble human ACE2 (sol-hACE2-Fc)	Laboratory of Stefan Pöhlmann	N/A
Lipofectamine 2000	Thermo Fisher Scientific	Cat# 11668019
Critical commercial assays
Beetle-Juice Kit	PJK	Cat# 102511
Deposited data
N/A	N/A	N/A
Experimental models: cell lines
293T	DSMZ	Cat# ACC-635; RRID: CVCL_0063
BHK-21	Laboratory of Georg Herrler	ATCC Cat# CCL-10; RRID:CVCL_1915
Caco-2	Laboratory of Stefan Pöhlmann	ATCC Cat# HTB-37; RRID: CVCL_0025
Calu-3	Laboratory of Stephan Ludwig	ATCC Cat# HTB-55; RRID: CVCL_0609
Huh-7	Laboratory of Thomas Pietschmann	JCRB Cat# JCRB0403; RRID: CVCL_0336
NCI-H1299	Laboratory of Stefan Pöhlmann	ATCC Cat# CRL-5803; RRID:CVCL_0060
Vero76	Laboratory of Andrea Maisner	ATCC Cat# CRL-1586; RRID: CVCL_0574
Experimental models: organisms/strains
N/A	N/A	N/A
Oligonucleotides
Please see Table S3	Sigma-Aldrich	N/A
Recombinant DNA
Plasmid: pCG1	Laboratory of Roberto Cattaneo	N/A
Plasmid: pCAGGS-VSV-G	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCAGGS-DsRed	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (B.1), codon-optimized	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (B.1.351), codon-optimized	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (B.1.621), codon-optimized	This study	N/A
Plasmid: pCG1-SARS-2-SΔ18 (C.1.2 “early”), codon-optimized	This study	N/A
Plasmid: pCG1-SARS-2-SΔ18 (C.1.2 “late”), codon-optimized	This study	N/A
Plasmid: pCG1-solACE2-Fc	Laboratory of Stefan Pöhlmann	N/A
Software and algorithms
Hidex Sense Microplate Reader Software	Hidex Deutschland Vertrieb GmbH	https://www.hidex.de
YASARA (version 19.1.27)	YASARA Biosciences GmbH	http://www.yasara.org
SWISS-MODEL online tool	Protein Structure Bioinformatics Group, Swiss Institute of Bioinformatics Biozentrum, University of Basel	https://swissmodel.expasy.org
Adobe Photoshop CS5 Extended (version 12.0 x 32)	Adobe	https://www.adobe.com/
GraphPad Prism (version 8.3.0(538))	GraphPad Software	https://www.graphpad.com/
FloJo (version 10.8)	Becton Dickinson	https://www.flowjo.com/
Flowing Software (version 2.5.1)	Turku Bioscience	https://bioscience.fi/services/cell-imaging/flowing-software/
Adobe Photoshop CS5 Extended (version 12.0 x 32)	Adobe	https://www.adobe.com/
Microsoft Office Standard 2010 (version 14.0.7232.5000)	Microsoft Corporation	https://products.office.com/
Other
Complex of SARS-CoV-2 receptor binding domain with the Fab fragments of two neutralizing antibodies (PDB: 6XDG)	(Hansen et al., 2020)	https://www.rcsb.org/structure/6XDG
SARS-CoV 2 Spike Protein bound to LY-CoV555 (PDB: 7L3N)	(Jones et al., 2021)	https://www.rcsb.org/structure/7L3N
Molecular basis for a potent human neutralizing antibody targeting SARS-CoV-2 RBD (PDB: 7C01)	(Shi et al., 2020)	https://www.rcsb.org/structure/7C01
Distinct conformational states of SARS-CoV-2 spike protein (PDB: 6XR8)	(Cai et al., 2020)	https://www.rcsb.org/structure/6XR8