Fig. 1. AML-IRF7^−/− mice exhibit accelerated disease progression.

A Schematic overview of the development of the MLL-AF9-induced AML-WT and AML-IRF7^−/− mouse models. B–F The disease progression of the two models was studied when 1 × 10⁶ AML cells were transplanted into recipient mice. B The percentage of GFP⁺ cells in peripheral blood, spleen, and bone marrow was monitored every 5 days (n = 4). C The survival of the mice is shown in Kaplan–Meier curves (n = 10 for each group). D, E The mice were sacrificed on day 15. The size and weight of the spleens (D) and livers (E) are shown, and HE-stained sections of bone marrow, spleen, liver, brain, and kidney were observed under a light microscope. F Scale bars, 100 μm. G–I The mice were transplanted with 1 × 10⁶ AML cells followed by Ara-C treatments at the indicated time points. G Schematic overview of the experiments. H The percentage of GFP⁺ cells in peripheral blood was monitored on days 8, 12, 16, and 20. I The survival of the mice is shown in Kaplan–Meier curves (n = 10 for each group). Data are presented as mean ± S.E.M. Unpaired Student’s t test, one-way ANOVA tests and Kaplan–Meier estimates were used. ***p < 0.001.