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. 2022 Mar 7;41(16):2303–2314. doi: 10.1038/s41388-022-02233-w

Fig. 6. Blocking VCAM1-VLA-4 axis delays disease progression and attenuates intracerebral invasion in AML-IRF7−/− mice.

Fig. 6

A, B An equal number of AML-WT or AML-IRF7−/− cells were transplanted into the mice on day 0 and the mice were sacrificed when peripheral blood GFP+ cells reached 10% (A) and 20% (B). Representative results of HE-stained brain sections are shown. Scale bars, 100 or 50 μm. C An equal number of THP1sc or THP1sh1 cells were transplanted into the NSG mice on day 0 and the mice were sacrificed on day 25. Representative results of HE-stained brain sections are shown. Scale bars, 100 or 50 μm. D The expression of genes was analyzed by qRT-PCR. E The expression of VCAM1 in AML cells was analyzed by flow cytometry. Representative results are shown (left), and the percentage of VCAM1+ cells is plotted (right). F, G The mice were intravenously injected with an equal number of AML-IRF7−/− cells followed by daily administration with or without firategrast. The percentage of peripheral blood AML cells was monitored every 5 days (n = 5) (F), and the survival of mice is shown in Kaplan–Meier curves (n = 10) (G). H The mice were intravenously injected with an equal number of AML-IRF7−/− cells followed by daily administration of firategrast or not. The mice were sacrificed when PB GFP+ cells reached 20%. Representative results of HE-stained brain sections are shown. Scale bars, 100 or 50 μm. Data are presented as mean ± S.E.M. Unpaired Student’s t test, one-way ANOVA tests and Kaplan–Meier estimates were used. ***p < 0.001.