Fig. 2.

TRPV1 and UCP1 double knockout attenuated energy metabolism. A Oxygen consumption in the light (left panel) and dark (right panel) of WT, TRPV1^−/−, UCP1^−/− and TRPV1^−/−/UCP1^−/− mice was measured in metabolism cages. B, C Heat production (B) and 24-h locomotor activities (C) of the four groups of mice were measured in metabolism cages. E, F Twenty-four-hour ambulatory core body temperatures (E) and 24-h mean basal body temperatures (F) in mice were measured by the telemetry system at 25 °C ambient temperature. G Rectal temperatures of WT, TRPV1^−/−, UCP1^−/− and TRPV1^−/−/UCP1^−/− mice were measured in the anus of mice by thermometer at 8 AM every day at 25 °C ambient temperature. The values are shown as the means ± SEM; n = 6 per group; *P < 0.05, **P < 0.01 vs. WT mice; ^#P < 0.05, ^##P < 0.01 vs. TRPV1^−/− mice; ^∆P < 0.05, ^∆∆P < 0.01 vs. UCP1^−/− mice