Fig. 5.

TRPV1 and UCP1 double knockout impaired mitochondrial ion balance. A Mitochondrial [Ca²⁺] of primary cultures of brown adipocytes from WT, TRPV1^−/−, UCP1^−/− and TRPV1^−/−/UCP1^−/− mice was measured after loading with Rhod2 (2 μmol/L). The lower panel is the bar graph derived from the upper figure with [Ca²⁺] at 20 μM. B, C Immunoblots of TRPV1, UCP1, and LETM1 (B); ACC (P79); and ACC (C) in the BAT of WT, TRPV1^−/−, UCP1^−/− and TRPV1^−/−/UCP1^−/− mice. Values are presented as the means ± SEM for five experiments (the third row corresponds to the panel of the second row). *P < 0.05, **P < 0.01 vs. WT mice; ^#P < 0.05, ^##P < 0.01 vs. TRPV1^−/− mice; ^∆P < 0.05, ^∆∆P < 0.01 vs. UCP1^−/− mice