Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 9;41(16):2372–2389. doi: 10.1038/s41388-022-02253-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Representative immunofluorescence images of advanced pancreatic cancer biopsies before and after chemotherapy. Arrows indicate iCAFs: PDPN ⁺IL6⁺. Scale bar = 50 μm. Quantification of the presence of iCAFs (B). Panc-1 and MIAPaCa-2 cells were cultured under CAF^S1-CM or CAF^R1-CM for 3 days and then subjected to the indicated experiments. C–E Cells were treated with oxaliplatin for 48 h. Cell viability was measured by CCK-8 and the IC50 value was calculated. F, G Colony formation assay and (H, I) flow cytometry apoptosis analyses were performed to evaluate the chemoresistance of pancreatic cancer cells in each group. J Volcano plot of differentially expressed genes from the mRNA transcriptomes of CAF^S1-CM and CAF^R1-CM. Three biological replicates of each CAF cell line were used for the RNA sequencing. K Expression level of specific genes in CAF^S1-CM and CAF^R1-CM. L GSEA of significantly upregulated pathways in CAF^R1 compared with CAF^S1. The results are presented as the mean ± SD of three technical replicates. *P < 0.05; **P < 0.01; ***P < 0.001, ns no significance.