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. 2022 Mar 9;41(16):2372–2389. doi: 10.1038/s41388-022-02253-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Representative FISH images showing the cellular localization of UPK1A-AS1. The UPK1A-AS1 probe was labeled with Cy3 (red), and the nuclei were stained with DAPI (blue). Scale bar = 10 μm. B qRT-qPCR analysis following subcellular fractionation of UPK1AS-AS1. C Silver staining of UPK1A-AS1-associated nuclear proteins. Two specific bands (arrow) were excised and subjected to mass spectrometry (D) MS identification of UPK1A-AS1-binding proteins. E Western blot analysis of Ku70 and Ku80 using protein samples enriched by biotinylated UPK1A-AS1 sense and antisense RNAs. F Fold enrichment of UPK1A-AS1 in RNA samples precipitated with Ku70, Ku80 or IgG antibody in Panc-1 cells. G Western blot analysis of Ku70 and Ku80 using protein samples enriched by serial deletions of UPK1A-AS1. H RNAalifold predicted the secondary structure of UPK1A-AS1. The insects indicated Ku70 and Ku80 binding stem-loop structures in UPK1A-AS1. I Fold enrichment of UPK1A-AS1 in RNA samples precipitated with Ku70, Ku80 or IgG antibody after site-directed mutagenesis of region A and region B of UPK1A-AS1 in Panc-1 cells. The results are presented as the mean ± SD of three technical replicates. **P < 0.01; ***P < 0.001.