REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CD4-APC, clone OKT4	BioLegend, San Diego, U.S.	Cat. no. 317416; RRID:AB_571945
anti-CD127-FITC, clone eBioRDR5	Thermo Fisher Scientific, Waltham, U. S.	Cat. no. 11-1278-42; RRID:AB_1907342
anti-CD25-PE, clone M-A251	BioLegend, San Diego, U.S.	Cat. no. 356104; RRID:AB_2561861
anti-FOXP3-BV421, clone 206D	BioLegend, San Diego, U.S.	Cat. no. 320123; RRID:AB_2561338
H3K4me3	Active Motif, Carlsbad, U.S.	Cat. no. 39915; RRID:AB_2687512
H3K27ac	Diagenode	Cat. no. C15410196; RRID:AB_2637079
Biological samples
Lymphocyte cones were obtained with informed consent from healthy adults of Caucasian origin.	NHS Blood and Transplant, Cambridge and from the NHS Blood and Transplant, Oxford	REC 15/NW/0282, REC 15/NS/0060
Chemicals, peptides, and recombinant proteins
TRIzol	Thermo Fisher Scientific	15596026
NEBNext® High-Fidelity 2X PCR Master Mix	New England Biolabs, Ipswich, U.S.	M0541L
Tn5 enzyme	Nextera	TDE1
EvaGreen dye	Biotium, Fremont, U.S.	#31000
Critical commercial assays
EasySep® Human CD4+ T Cell Enrichment Kit	StemCell Technologies, Vancouver, Canada	Cat. no. 19052
iDeal ChIP-seq Kit for Histones	Diagenode, Liege, Belgium	C01010059
RNeasy Mini Kit	QIAgen, Hilden, Germany	74106
KAPA RNA HyperPrep Kit	Roche, Basel, Switzerland	KK8541
Nextera DNA Library Prep Kit	Illumina, U.S.	FC-131-1096
MinElute PCR Purification Kit	QIAgen, Hilden, Germany	28006
Nextera Index Kit	Illumina, U.S.	TG-131-2001
Deposited data
Raw data generated in this study	EGA	https://wwwdev.ebi.ac.uk/ega/studies/EGAS00001003516
BLUEPRINT consortium CD4+ T cell and monocyte RNA-seq and ChIP-seq datasets	EGA	EGAD00001002671, EGAD00001002674, EGAD00001002673, EGAD00001002674
DICE project data	DICE project: Linking immune disease GWAS variants to genes and cell types, Date of approval: 2019-08-23	https://dice-database.org/
FANTOM5	Predefined enhancer-TSS bed sets	http://enhancer.binf.ku.dk/presets/enhancer_tss_associations.bed
Custom scripts and pipelines repository:	Treg_Multiomic	https://github.com/trynkaLab/
Software and algorithms
GitHub (original codes supporting this work)	https://doi.org/10.5281/zenodo.6335757	https://github.com/TrynkaLab/Treg_Multiomics/tree/v1.0.1
BEAGLE 4.1	Browning et al.60	http://faculty.washington.edu/browning/beagle/beagle.html
VerifyBamID v1.0.0	Jun et al.61	https://github.com/statgen/verifyBamID/releases
STAR	Dobin et al.62	https://github.com/alexdobin/STAR/releases
subread package v1.5.1	Liao et al.63	http://subread.sourceforge.net/
skewer	Jiang et al.64	https://github.com/relipmoc/skewer
bwa	Li and Durbin.65	http://bio-bwa.sourceforge.net/
samtools	Li et al.66	http://samtools.sourceforge.net/
MACS2	Zhang et al.67	https://github.com/macs3-project/MACS
BEDTOOLS	Quinlan and Hall68	https://bedtools.readthedocs.io/en/latest/
QTLtools	Delaneau et al.69	https://qtltools.github.io/qtltools/
coloc v2.3–1	Giambartolomei et al.31	https://github.com/chr1swallace/coloc
TFmotifView	Leporcq et al.70	http://bardet.u-strasbg.fr/tfmotifview/
g:Profiler	Raudvere et al.66	https://biit.cs.ut.ee/gprofiler/gost
DESeq2_.1.14	Love et al.71	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
ASEReadCounter (4.0.1.1)	Castel et al.72	https://gatk.broadinstitute.org/hc/en-us/articles/360037054312-ASEReadCounter
Other
Lympholyte-H density gradient centrifugation.	(Cedarlane Labs, Burlington, Canada)	CL5020
Infinium® CoreExome-24 v1.1 BeadChip	Illumina	WG-331-1101