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. 2022 Apr 14;8:200. doi: 10.1038/s41420-022-00996-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A qRT-PCR was used to determine changes in stem cell regulatory factors after SNORD1C knockdown or overexpression. B Western blotting was used to detect CD44 expression after SNORD1C knockdown. C Western blotting was conducted to compare SOX2 expression between adherent and spheroid SW620 cells. D qRT-PCR was used to compare SNORD1C expression between adherent and spheroid CRC cells. E Images of sphere formation in SNORD1C-knockdown and -overexpressing CRC cells. F Resistance to 5-FU in SNORD1C-knockdown and -overexpressing CRC cells.