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. 2022 Apr 14;9:10. doi: 10.1038/s41439-022-00188-8

Fig. 5. Expression of human ATXN7 (wildtype and mutant variants) and human TOP1MT (wildtype and mutant variants) in the Drosophila central nervous system has variable effects on locomotor activity and lifespan.

Fig. 5

A Schematic representation outlining the development of transgenic flies prior to experimentation. Briefly, we used the pUASTattb vector system which allows for PhiC31-mediated transgenesis. We crossed flies expressing ataxin-7 Q35P and ataxin-7 N556K together with elav-GAL4 with those expressing top1mt R111W to obtain relevant genotypes. B Climbing indexes for all control and experimental genotypes described were obtained at one, three, and five weeks of age, with at least six trials per genotype per time point. Experiment was repeated three times. Error bars represent standard error. C Kaplan–Meier survival curves for lifespan. Experiment was repeated three times and results combined in the depicted graph. N = 200 flies per genotype. Figure created with BioRender.com.