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. 2022 Apr 14;8:202. doi: 10.1038/s41420-022-00909-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A mRNA expression of GSDMD in myocardial tissues of mice with I/R detected by RT-qPCR, normalized to GAPDH; B Representative images of GSDMD and GSDMD N protein bands in myocardial tissues of mice with I/R. C Western blot analysis of GSDMD protein and GSDMD N in myocardial tissues of mice with I/R, normalized to GAPDH; D Immunohistochemistry staining of GSDMD protein in myocardial tissues of mice with I/R. N = 10 for mice in each group. Measurement data were expressed as mean ± standard deviation. Data comparison between two groups was conducted by unpaired t-test. *p < 0.05 vs. sham-operated mice.