FIGURE 2.

Hickory phylogenetic tree constructed with 424 plant TLP genes (A) and full-length peptides sequences of 40 and three TLPs from hickory and three Arabidopsis thaliana, respectively (B). The phylogenetic tree of 424 plant TLP genes was plotted with the maximum-likelihood estimation, JTT + R7 substitution model, and 1,000 bootstrap times. The phylogenetic tree was divided into five clades: Purple, dark red, blue, orange, and green. The 43 TLP peptides sequences were aligned using the MAFFT software. Then, the phylogenetic tree was inferred with IQTree with the parameters of the JTT + I + G4 substitution model and 1,000 times bootstrap. The maximum-likelihood tree could be divided into three groups, presented by red, green, and blue lines.