Figure 2.

AAV5 and AAVzk2 capsids were post-translationally modified and exhibited differential PTM patterns

(A) A schematic workflow for mass spectrometry-based PTM analysis of AAV5 and AAVzk2 capsids. (B) PTM residue positions along the capsid sequences of the AAV5 and AAVzk2 from the N to the C terminus. Different colors represent different PTMs (red, acetylation; blue, methylation; orange, ubiquitination; purple, phosphorylation; light blue, deamidation; violet, O-glycosylation). The inserted sequence FAPTPGP in AAVzk2 capsid is highlighted in the gray box. (C) Common and unique capsid PTMs for AAV5 and AAVzk2 capsids. The color and its corresponding PTMs were the same as those in (B). (D) The number of the individual PTM site detected in AAV5 and AAVzk2 capsids.