Figure 4.
Selective GFP mRNA and protein expression in the livers of AAVzk2-treated mice
(A) Male C57BL/6 mice were injected via tail vein with the indicated AAV serotypes encapsulating GFP transgene at a dose of 1 × 1013 vg/kg, and liver, heart, lung, spleen, kidney, and brain were subjected to qRT-PCR to detect GFP expression, in which the GFP mRNA levels of the AAV8, AAV9, and AAVzk2 groups were normalized to the AAV5 group. n = 4 mice per group. (B) Western blot to detect GFP protein expression in the livers and hearts from the mice treated with the indicated AAVs. (C) Quantification of GFP protein levels in the liver relative to the heart. n = 4 male and 4 female mice per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, One-way ANOVA.