Figure 6.
Robust human hepatocellular transduction property of AAVzk2
(A) Human hepatocellular carcinoma cell Huh7 and normal liver cell L02 were infected by the indicated AAV particles encapsulating the GFP gene at a multiplicity of infection (MOI) of 1 × 105 and 1 × 104 vg/cell, respectively. Images were captured 48 h post-virus treatment. Upper panel, GFP signal; lower panel, bright field. Scale bar, 100 μm. (B) Quantification of GFP-positive cell percentage. The ratios of GFP signal area of Huh7 and L02 cells to the whole bright-field area were quantified at an MOI of 1 × 105 and 1 × 104 vg/cell, respectively. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. n = 6 wells of cells. One-way ANOVA. (C) Lysates of Huh7 and L02 cells treated with the indicated AAV particles at an MOI of 1 × 105 vg/cell were subjected to immunoblotting for GFP. Three lanes were performed for each AAV-treated group. (D) Quantification of relative GFP protein levels in (C). ∗∗p < 0.01, ∗∗∗p < 0.001. n = 4 wells of cells. One-way ANOVA.