Figure 2.

miR-378 overexpression exaggerated ACLT-induced OA

(A and B) Safranin O and fast green staining (A) and OARSI scores (B) of knee joint cartilage collected from both WT and miR-378 TG mice at weeks 3 and 6 post ACLT + DMM surgery (n = 8, ∗∗∗p < 0.001). Scale bars: A1 and A2, A5 and A6, 200 μm; A3 and A4, A7 and A8, 100 μm. (C–E) IHC staining and semi-quantitative analysis (integrated optical density[IOD])/μm²)] of the percentage of Mmp13- (C and D) and Col II (C and E)-positive chondrocytes in articular cartilage from two group of mice at weeks 3 and 6 post ACLT + DMM surgery. Sections were counterstained with hematoxylin. Scale bar, 100 μm (n = 8; ∗∗∗p < 0.001).