Figure 3.

miR-378 exaggerates inflammatory response by repressing autophagy in OA chondrocytes

(A–E) The mRNA expression levels of inflammatory makers iNos (A) and Cox2 (B); chondrogenesis marker Col2a1 (C), chondrocyte hypertrophy marker Mmp13 (D), and autophagy marker Beclin-1 (E) of WT and miR-378 chondrocytes under normal or inflammatory condition were assessed by real-time PCR (n = 6; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (F and G) The protein expression levels of iNos, Cox2, Col II, and Mmp13 (F) as well as Beclin-1, LC3-I, and LC3-II (G) of WT and miR-378 chondrocytes under normal or inflammatory conditions were determined by western blot analysis. (H) Fluorescence images of Cyto-ID dye-stained WT and miR-378 chondrocytes under normal and inflammation conditions. Scale bar, 100 μm (n = 6).