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. 2022 Mar 23;28:328–341. doi: 10.1016/j.omtn.2022.03.016

Figure 4.

Figure 4

miR-378 repressed BMSC chondrogenesis activity

(A) Safranin O, Alcian blue, and IHC staining using COL II antibody of WT and miR-378 BMSC micromass after chondrogenesis induction. Scale bar, 400 μm. (B–F) The mRNA expression levels of chondrogenesis marker Sox9 (B), Col2a1 II (C), Acan (D), chondrocyte hypertrophy marker Col X (E), and Mmp13 (F) in WT and miR-378 BMSCs during chondrogenesis were evaluated by real-time PCR (n = 6; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (G) Protein expression levels of Col II, Acan, and Mmp13 in BMSCs after 21-day chondro-induction were revealed by western blot analysis.