Figure 7.

Anti-miR-378-mediated BMSCs ameliorated ACLT-induced OA

(A and B) Safranin O and fast green staining (A) and OARSI scores (B) of knee joint cartilage collected from miR-378 TG mice received anti-miR-378-3p incorporated or negative control lentivirus injection at week 4 post ACLT + DMM surgery (n = 8; ∗∗p < 0.01). Scale bars, A1 and A3, 200 μm; A3 and A4, 100 μm (A). (C–G) IHC staining (C) and quantitative analysis of the percentage of Col II- (D), Mmp13- (E), Atg2a- (F), and Sox6 (G)-positive chondrocytes in articular cartilage from two groups of mice at week 4 post ACLT + DMM surgery (n = 8; ∗p < 0.05, ∗∗p < 0.01). Sections were counterstained with hematoxylin. Scale bars, 50 μm.