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. 2022 Apr 1;10:837486. doi: 10.3389/fcell.2022.837486

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Copyright © 2022 Phyo, Uchida, Chen, Caporizzo, Bedi, Griffin, Margulies and Prosser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total tubulin content increases and the microtubule network densifies during hypertrophy. Representative immunoblots and relative fold-change of α-tubulin (left) and β-tubulin (right) in the (A) total proteins, (B) free, or (C) polymerized -tubulin fractions (n = Ctrl:7, PE:7, Iso:6). Representative immuno- blots with technical duplicate lanes and relative fold-change using GAPDH (left) or α-tubulin (right) as loading controls for (D) acetylated α-tubulin, (E) detyrosinated α-tubulin, (F) Δ2 α-tubulin, (G) polyglutamylated α-tubulin, & (H) polyglycylated pan-tubulin (n = Ctrl:7, PE:7, Iso:6). For all bar plots, whiskers represent + 1SEM and bar represents mean; * represents p-value from Welch-corrected two-tailed two-sample t-test < 0.025 (Bonferroni-corrected for two comparisons), ** represents p < 0.01, and *** represents p < 0.001.