Table 1.

Expression of genes related to methionine metabolism in methionine-addicted cancer cells and their methionine-independent revertants

Group	Protein	Gene name	HCT 116		H460
			log2 Fold Change (revertant/parent)	p value	log2 Fold Change (revertant/parent)	p value
Methionine synthase and related proteins	Methionine synthase	MTR	0.00	0.99	−0.17	0.50
	Methionine synthase reductase	MTRR	−0.02	0.91	0.21	0.41
AdoMet synthetases		MAT1A	−1.50	0.27	0.70	0.66
		MAT2A	−0.52	0.31	0.93	0.01
	Regulatory subunit for MAT2A	MAT2B	0.09	0.49	0.14	0.57
Folate cycle enzymes that support methionine synthesis	Serine transhydroxylmethylase (cytoplasmic)	SHMT1	−0.04	0.76	−0.01	0.98
	Serine transhydroxymethyltransferase (mitochondrial)	SHMT2	0.04	0.73	−0.56	0.06
	methylene-THF reductase	MTHFR	−0.37	0.12	−0.27	0.40
	Folate receptors	FOLR1	−1.06	0.02
	Thymidylate synthase	TYMS	0.18	0.19	0.29	0.28
Methionine salvage and polyamine biosynthesis	Methylthioadenosine phosphorylase	MTAP	0.04	0.74	0.08	0.74
	AdoMet decarboxylase	AMD1	−0.21	0.14	0.60	0.05
	Spermidine synthase	SRM	0.19	0.19	0.13	0.61
	Spermine synthase	SMS	0.10	0.45	0.06	0.79
AdoMet sensors		BMT2	0.27	0.22	−0.37	0.26
Homocysteine metabolism	Adenosylhomocysteine hydrolase	AHCY	0.04	0.73	−0.13	0.59
		AHCYL1	−0.13	0.35	−0.15	0.54
		AHCYL2	0.01	0.93	−0.52	0.08
	Cystathionine beta synthase	CBS			−0.30	0.50
		CBSL			−0.52	0.31
	Cystathionine gamma lyase	CTH	−0.15	0.50	−0.75	0.03
Glycine metabolism	Sarcosine dehydrogenase	SARDH	−0.31	0.22	0.58	0.59
	Dimethylglycine dehydrogenase	DMGDH	−0.12	0.88