Fig. 5.

Substitution frequency change before and after treatment in NS5A.

The sFC relative to the reference amino acids before and after treatment was calculated independently at each of the first 200 amino acids within NS5A. The dotted line represents the mean sFC across the 200 amino acids in each genotype. The gray bar across the amino acids depicts a "genetic drift corridor" (2 SD from the mean sFC) to exclude variants with usual genetic drifts. Amino acid positions with a significant sFC before and after treatment are shown. A "positive" sFC represents an increase in the substitution levels (or decrease in the reference amino acid levels) after treatment; a "negative" sFC represents a decrease in the substitution levels (or increase in the reference amino acids) after treatment. Amino acid positions with a high-frequency change but without a specific substitution (i.e., only a mixture of substitutions) are not indicated. The amino acids of interest identified by the pairwise comparison are also noted (ˆ) for comparison. GT, genotype; sFC, substitution frequency change.