Table 6.
Primer sequences of i5 and i7 index primers
| Index name | Primer sequence 5′-3′ | |
|---|---|---|
| i5 | S513 | AATGATACGGCGACCACCGAGATCTACAC[TCGACTAG]TCGTCGGCAGCGTC |
| S515 | AATGATACGGCGACCACCGAGATCTACAC[TTCTAGCT]TCGTCGGCAGCGTC | |
| S516 | AATGATACGGCGACCACCGAGATCTACAC[CCTAGAGT]TCGTCGGCAGCGTC | |
| S517 | AATGATACGGCGACCACCGAGATCTACAC[GCGTAAGA]TCGTCGGCAGCGTC | |
| S518 | AATGATACGGCGACCACCGAGATCTACAC[CTATTAAG]TCGTCGGCAGCGTC | |
| S520 | AATGATACGGCGACCACCGAGATCTACAC[AAGGCTAT]TCGTCGGCAGCGTC | |
| S521 | AATGATACGGCGACCACCGAGATCTACAC[GAGCCTTA]TCGTCGGCAGCGTC | |
| S522 | AATGATACGGCGACCACCGAGATCTACAC[TTATGCGA]TCGTCGGCAGCGTC | |
| i7 | N716 | CAAGCAGAAGACGGCATACGAGAT[TAGCGAGT]GTCTCGTGGGCTCGG |
| N718 | CAAGCAGAAGACGGCATACGAGAT[GTAGCTCC]GTCTCGTGGGCTCGG | |
| N719 | CAAGCAGAAGACGGCATACGAGAT[TACTACGC]GTCTCGTGGGCTCGG | |
| V720 | CAAGCAGAAGACGGCATACGAGAT[AGGCTCCG]GTCTCGTGGGCTCGG | |
| N721 | CAAGCAGAAGACGGCATACGAGAT[GCAGCGTA]GTCTCGTGGGCTCGG | |
| N722 | CAAGCAGAAGACGGCATACGAGAT[CTGCGCAT]GTCTCGTGGGCTCGG | |
| N723 | CAAGCAGAAGACGGCATACGAGAT[GAGCGCTA]GTCTCGTGGGCTCGG | |
| N724 | CAAGCAGAAGACGGCATACGAGAT[CGCTCAGT]GTCTCGTGGGCTCGG | |
| N726 | CAAGCAGAAGACGGCATACGAGAT[GTCTTAGG]GTCTCGTGGGCTCGG | |
| N727 | CAAGCAGAAGACGGCATACGAGAT[ACTGATCG]GTCTCGTGGGCTCGG | |
| N728 | CAAGCAGAAGACGGCATACGAGAT[TAGCTGCA]GTCTCGTGGGCTCGG | |
| N729 | CAAGCAGAAGACGGCATACGAGAT[GACGTCGA]GTCTCGTGGGCTCGG | |
Sequences of i5 and i7 bases are bold and in square brackets.