Figure 4.

Disease classification based on gut microbiome signature. (A) Classification performance of the random forest model using the relative abundance of NAC- efficacy associated genera was assessed by the area under the ROC in BC patients. The combination of 3 signature bacteria: Coprococcus, Dorea, and uncultured Ruminococcus sp. The combination of 9 optimal species markers: Bacteroides, Coprococcus, Dorea, Fusicatenibacter, Ruminococcus, Butyrivibrio sp. CAG 318, Lactobacillus salivarius, Bacteroides xylanisolvens, and uncultured Ruminococcus sp. (B) Identification of the signature gut microbiota associated with NAC- efficacy by random forest. Fivefold cross-validation together with random forest was performed to determine the signature biomarkers. Detailed signature biomarkers’ random seed from the random forest is presented between the NAC non-effectual group and the NAC effectual group.