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. 2022 Apr 1;13:794327. doi: 10.3389/fendo.2022.794327

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Copyright © 2022 Karvonen, Krohn, Ranki and Hau

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferation in APECED iPS-derived EBs is seen along the cortical layers and apoptosis within the medulla creating a lumen. Top panel: Proliferative (Ki-67, brown) and apoptotic (TUNEL, brown) cells in EBs generated from iPS cells of one healthy control HEL24.3 (first column and third column), and in two APECED iPS cell lines 137.2 (second column) and 138.6 (fourth column) as detected by Ki-67 and TUNEL immunocytochemistry, respectively. The FFPE samples were collected on days 3, 7 and 30 after generation of the EBs. Mayer’s hematoxylin (blue) as nuclear counterstain. Bottom graph: Quantitation of proliferation (Ki-67+) and apoptosis (TUNEL+) in the EBs reveals they are similar irrespective of AIRE mutation. Error bars represent SD.