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. 2022 Apr 1;13:794327. doi: 10.3389/fendo.2022.794327

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Copyright © 2022 Karvonen, Krohn, Ranki and Hau

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AIRE mutation does not affect the spatial localization of AIRE, OCT4, or NANOG proteins in EBs. Panel (A) AIRE (green staining, indicated with white arrow heads) and OCT4 (red staining, indicated with black arrow heads) protein expression in EBs generated from iPS cells of APECED patient 138.6 and healthy control HEL24.3 on day 3, 7 and 30 after the generation of EBs. Panel (A) images are without Mayer’s hematoxylin counterstaining. Panel (B) AIRE (green staining, indicated with white arrow heads) and NANOG (red staining, indicated with black arrow heads) protein expression detected by immunocytochemistry in EBs generated from iPS cells of APECED patient 137.2 and healthy control HEL24.3. FFPE samples collected on days 3, 7 and 30 after the generation. Mayer’s hematoxylin (blue) used as a nuclear counterstain on images on the first and third rows. Images on second and fourth row are without Mayer’s hematoxylin. Scale bar equals 50 µm. Panel (C) Quantification of positive cells in AIRE + OCT4 and AIRE + NANOG immunostainings shown in panels (A, B). Error bars represent SD.