Phusion GC buffer, 5x	10 µl
dNTPs (10 mM)	0.5 µl
gRNA-fw2 (100 µM)	0.35 µl
DR274-rev (100 µM)	0.35 µl
gRNA oligo (100 µM)	0.25 µl
Constant sequence (100 µM)	0.02 µl
Phusion DNA polymerase (2 U/µl)	0.5 µl
Nuclease-free water	38.03 µl
	50 µl