CAS9-NLS protein (PNA Bio, cat# CP03), reconstituted in 1× CAS9 buffer to 5 mg/ml	1 μl
Guide RNA (4 μg)	x μl
CAS9 buffer, 10× (200 mM HEPES, 1.5 M KCl, pH 7.5)	0.9 μl
Nuclease-free water	make up to 10 µl