Gene expression profile by RNA-sequencing of hVICs cultured with or without Gal-3. (A) Volcano map of DEGs in C versus Gal-3 [log10 (Gal-3/C); up-regulation: 79 genes and down-regulation: 82 genes]; (B) heatmap for common DEGs with group clusters; (C) KEGG pathway enrichment, where orange dots indicate the degree of enrichment [Q value (−log10)]; (D) transcription factor enrichment, yellow (deep) color indicates the degree of enrichment [Q value (−log10)]. Gal-3, galectin-3; hVICs, human aortic valve interstitial cells; DEGs, differentially expressed genes.