TABLE 4.

Primers used for dsRNA synthesis and RT-qPCR.

Gene name	Primer	Sequence 5′–3′	Amplicon (bp)	Amplification efficiency (%)
dsvATPase A	Fw	TAATACGACTCACTATAGGGAGATATCCAGCGACCCCTGAAG	300	−
	RV	TAATACGACTCACTATAGGGAGATTAGTTTTCTCACCATCAAACTCTG
ds GFP	Fw	TAATACGACTCACTATAGGGAGAATGGTGAGCAAGGGCGAG	496	−
	RV	TAATACGACTCACTATAGGGAGATGTTCTGCTGGTAGTGGTCG
18S	Fw	GTGCTTTGCAGTGGTTGTGT	107	99.3
	RV	TCGGGCCGTTCGACTTAATG
60S	Fw	GCTCCCAAGATCGGTCCTCT	119	96.8
	RV	TGCCTGTTTTGAATAGTGAGGC
vATPase A	Fw	AATTGTGCAGCTGGTCGGTA	127	99.6
	RV	TGGGCAGAACCGATCGTAAG
Dcr-2	Fw	ACATTGCTGATGGAACGGGAT	84	104.9
	RV	AGGCTGTTTGGTCGACTTCC
Ago-2	Fw	TACGGCAGAGACCTCCATCA	102	102.6
	RV	GAGGAGGTCCTCTTTGTGCC

T7 Promoter sequence is underlined. Amplicon size is indicated, as well as primer efficiency when calculated.