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. 2022 Mar;10(6):344. doi: 10.21037/atm-22-982

Figure 3.

Figure 3

HIPK3 as a target gene of miR-146a. A The binding site between miR-146a and HIPK3 3’UTR. B. A dual-luciferase reporter gene assay was conducted to assess the interaction between miR-146a and HIPK3. (C) Western blotting analysis of HIPK3 expression in TGF-β-induced thymocytes transfected with NC, miR-146a inhibitor, or miR-146a mimics, respectively. (D) After transfection with miR-146a inhibitor or miR-146a mimics, the changed HIPK3 expression was monitored by RT-qPCR in TGF-β-induced thymocytes. *P<0.05, **P<0.01. NC, negative control; HIPK3, homeodomain-interacting protein kinases 3; TGF-β, transforming growth factor-β; RT-qPCR, reverse transcription quantitative polymerase chain reaction.