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. 2022 Mar;10(6):285. doi: 10.21037/atm-22-604

Figure 1.

Figure 1

Nrf2 knockdown regulated the expression of NLRP3 inflammasome under Q-VD-OPH-induced ischemic conditions. The cerebral cortex of rats was infected by lentiviral vectors containing Nrf2 green fluorescent protein (GFP) for 14 d and then subjected to Q-VD-OPH and cerebral I/R injury. (A) The transfected cerebral cortex of groups cont-sh RNA and sh Nrf2 was detected by green fluorescence under fluorescence microscope, at 10× magnification. Images were taken under identical conditions and exposures; (B) lentivirus-mediated Nrf2 knockdown was detected by quantitative RT-PCR and immunoblot; (C) the cerebral cortex infected by lentiviral vectors containing Nrf2 GFP was detected in the Nrf2-overexpression group by microscopy, with a magnification of 10; (D) lentivirus-mediated Nrf2 overexpression was detected by quantitative RT-PCR and immunoblot; (E) Western blot analysis of the NLRP3 inflammasome after Nrf2 knockdown; (F) Western blot analysis of the protein expression levels of NLRP3 inflammasome in the Nrf2-overexpression group. N=4 for each group. NS, no significant difference; *P<0.05. Nrf2, nuclear factor E2-related factor-2; NLRP3, NOD-like receptor family pyrin domain-containing 3.