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. 2022 Mar;10(6):304. doi: 10.21037/atm-22-556

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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m⁶A RNA methylation drives the downregulation of DBH-AS1 in PC. (A) GEPIA data revealed a positive correlation between DBH-AS1 and METTL3 expression levels in PC. (B) qPCR analysis of DBH-AS1 expression in PC cells treated with shMETTL3 or shControl. (C) The SRAMP database was utilized to detect m⁶A methylation loci within DBH-AS1. (D) MeRIP-PCR analysis of m⁶A-modified DBH-AS1. (E) MeRIP-PCR analysis of m⁶A-modified DBH-AS1 levels in cells treated with shControl or shMETTL3. (F) The impact of METTL3 on the degradation of DBH-AS1. (B,D-F) t-test. Data are means ± SEM from triplicate experiments, *, P<0.05. GEPIA, gene expression profiling interactive analysis database; PC, pancreatic cancer; qPCR, quantitative polymerase chain reaction; SRAMP, sequence-based RNA adenosine methylation site predictor; MeRIP, methylated RNA immunoprecipitation; sh, short hairpin.