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. 2022 Mar;10(6):304. doi: 10.21037/atm-22-556

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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DBH-AS1 inhibits the growth of PC cells. (A,C) qPCR analysis of PC cell growth following LV-DBH-AS1 LV-NC, shDBH-AS1, or shControl treatment. (B,D) PC cell colony formation was assessed for cells treated as in (A). The colonies were dyed with crystal violet and photographed under the camera (×1). (E,F) Nude mice subcutaneously injected with Canpan-2 cells stably transfected with LV-DBH-AS1 (n=5) or LV-NC (n=5). Subsequent analyses of tumor growth revealed that LV-DBH-AS1 inhibited in vivo PC tumor growth. Scale bar: 1cm. (A-D,F) t-test. Data are means ± SEM from triplicate experiments. *, P<0.05. PC, pancreatic cancer; qPCR, quantitative polymerase chain reaction; sh, short hairpin.