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. 2022 Mar;10(6):304. doi: 10.21037/atm-22-556

Figure 5.

Figure 5

DBH-AS1 sequesters miR-3163 and thereby promotes USP44 upregulation. (A) Schematic overview of complementary miR-3163 and DBH-AS1 sequences. (B) DBH-AS1 binding to miR-3163 assessed via dual-luciferase reporter assay. (C) Schematic overview of complementary miR-3163 binding sites within the USP44 3'-UTR. (D) miR-3163 binding to the USP44 3'-UTR assessed via dual-luciferase reporter assay. (E) USP44 mRNA levels measured in PC cells following miR-3163 mimic or mimic NC transfection. (F) USP44 mRNA levels were assessed in the indicated cells. (B,D,E) t-test; (F) variance test. Data are means ± SEM from triplicate experiments. *, P<0.05; ns, P>0.05. PC, pancreatic cancer; CCK-8, cell-counting kit 8; sh, short hairpin.