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Ivy1 localization relative to vacuoles by LLSM analysis after 3D deconvolution. Related to Fig. 2 D. Cells expressing mGFP-tagged Ivy1 with FM4-64-stained vacuoles were imaged for 1 min. The video shows 3D renderings (maximum-intensity projection mode in Imaris) of a deconvolved 3D time-lapse stack (see Materials and methods for details). The video was recorded at 8 frames/s; the original images were taken at 1.2-s time intervals.
